High prevalence of syphilis among recyclable waste collectors in Central Brazil

ABSTRACT Background: Syphilis is associated with social and behavioral factors. Methods: This cross-sectional study determined the prevalence of syphilis and its associated risk factors among recyclable waste collectors in Central Brazil. Results: A lifetime syphilis prevalence rate of 7.91% (95% confidence interval: 5.25-11.75) was found among 278 participants. Low educational level, history of sexually transmitted infection, and age ≥ 36 years were associated with a high prevalence of lifetime syphilis. Conclusions: These findings emphasize the need for syphilis prevention, screening, and treatment among recyclable waste collectors, highlighting the potential for the spread of infection in vulnerable populations.

PCR múltiplex para el diagnóstico de microorganismos atípicos transmitidos sexualmente / Multiplex PCR testing for the atypical microorganisms diagnosis sexually transmitted

Resumen Antecedentes: Las infecciones de transmisión sexual son un problema de salud pública mundial. El análisis rutinario incluye solo pruebas microbiológicas y serológicas para el diagnóstico de patógenos. Los microorganismos atípicos como Chlamydia trachomatis y micoplasmas no son identificados debido a los requerimientos. Además, no es incluida Gardnerella vaginalis, aunque se asocia a la vaginosis bacteriana. Objetivo: Desarrollar una PCR múltiplex para el diagnóstico de C. trachomatis, micoplasmas y G. vaginalis. Método: Se estandarizó la PCR múltiplex utilizando oligonucleótidos para C. trachomatis (gen ompA, orf6 plasmídico), Mycoplasma/Ureaplasma y G. vaginalis (genes rRNA16s). Resultados: Se estandarizaron pruebas de PCR múltiplex para los microorganismos estudiados, optimizándose las concentraciones y condiciones de las reacciones múltiplex. Se obtuvieron PCR dúplex para C. trachomatis (ompA, orf6), Chlamydia/Gardnerella y Chlamydia/micoplasmas y tríplex para Chlamydia/Mycoplasma/Ureaplasma. También un cuádruplex para Chlamydia/Mycoplasma/Ureaplasma/Gardnerella. Los resultados fueron verificados por PCR e hibridación automática (HybriSpot 12) y análisis in silico. Conclusión: Se desarrollaron pruebas de PCR múltiplex con una alta sensibilidad y especificidad para la identificación de C. trachomatis, micoplasmas y G. vaginalis.

Abstract Background: Sexually transmitted infections are a global public health problem. Routine analysis includes microbiological and serological tests for the diagnosis of pathogens. Atypical microorganisms such as Chlamydia trachomatis and mycoplasmas are not determined due to the requirements for their identification. Furthermore, Gardnerella vaginalis is not included despite being associated with bacterial vaginosis. Objective: To develop a multiplex PCR to diagnose Chlamydia, mycoplasmas, and Gardnerella. Method: Standardization of multiplex PCR tests was carried out using oligonucleotides for the identification of Chlamydia (ompA gene, plasmid orf6), Mycoplasma/Ureaplasma and Gardnerella (rRNA16s genes). Results: Multiplex PCR tests were standardized for the microorganisms studied, optimizing the concentrations and conditions of the multiplex reactions. Duplex PCR was obtained for Chlamydia (ompA, orf6), Chlamydia/Gardnerella, and Chlamydia/mycoplasmas, and triplex PCR for Chlamydia/mycoplasmas. Also, a quadruplex for Chlamydia, Mycoplasma/Ureaplasma and Gardnerella. PCR and automatic hybridization verified the results obtained (HybriSpot 12) and in silico analysis. Conclusion: Multiplex PCR tests with high sensitivity and specificity were developed to identify C. trachomatis, mycoplasmas, and G. vaginalis.

The relationship between HIV status and clinical manifestations of urogenital infections in women during pregnancy: a scoping review / A relação entre o status de HIV e as manifestações clínicas das infecções urogenitais em mulheres durante o período gravídico: uma revisão de escopo

Introduction: Maternal and child health are essential to public health, especially during pregnancy, where urogenital infections can affect mothers and fetuses. Sexually transmitted infections (STIs) increase obstetric risks and have complex connections with the human immunodeficiency virus ­ HIV. In Brazil, pregnant women with HIV are a growing concern, requiring focus and appropriate interventions. Objective: This study aimed to examine the clinical and epidemiological characteristics of urogenital infections in pregnant women with and without HIV and to assess whether there are notable differences between these groups. Methods: A scoping review was conducted following the Preferred Reporting Items for Systematic Reviews and Meta-Analyses ­ Extension for Scoping Reviews (PRISMA-ScR) and Joanna Briggs Institute guidelines. Databases such as Medical Literature Analysis and Retrieval System Online (MEDLINE), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), and Scientific Electronic Library Online (SciELO) were explored using relevant terms. Inclusion/exclusion criteria selected nine studies for analysis. A Population, Intervention, Comparison, Outcome, and Study Design (PICOS) approach directed the search. Results: Pregnant women with HIV had a high prevalence of STIs, including Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis, and bacterial vaginosis. HIV infection appears to influence the risk and severity of urogenital infections. Pregnancy increases the risk of STIs, regardless of HIV status. Male partners may also influence the presence of STIs in pregnant women, especially those with HIV. Conclusion: This study highlights the association between HIV status and urogenital infections in pregnant women, indicating the need for appropriate screening and care. Prevention and treatment of STIs in pregnant women are essential for maternal and child health, regardless of HIV status. An in-depth understanding of these issues can improve public policies, clinical practices, and preventive interventions that target the overall health of these vulnerable populations.Keywords: HIV. Signs and symptoms. Female urogenital diseases and pregnancy complications. Pregnant women. Sexually transmitted infections

Introdução: A saúde materna e infantil é essencial na saúde pública, especialmente durante a gravidez, quando infecções urogenitais podem afetar mães e fetos. Infecções sexualmente transmissíveis (IST) aumentam riscos obstétricos e têm conexões complexas com o vírus da imunodeficiência humana (HIV). No Brasil, gestantes com HIV são uma preocupação crescente, requerendo foco e intervenções adequadas. Objetivo: Este estudo teve como objetivo examinar as características clínicas e epidemiológicas das infecções urogenitais em mulheres grávidas com e sem HIV, avaliando se há diferenças notáveis entre esses grupos. Métodos: Uma revisão de escopo foi conduzida, seguindo as diretrizes Preferred Reporting Items for Systematic Reviews and Meta-Analyses - Extension for Scoping Reviews(PRISMA-ScR) e Joanna Briggs Institute. Bases de dados como Medical Literature Analysis and Retrieval System Online (MEDLINE), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), and Scientific Electronic Library Online (SciELO) foram exploradas com termos relevantes. Os critérios de inclusão/exclusão selecionaram nove estudos para análise. Uma abordagem do tipo População, Intervenção, Comparação, Desfecho e Desenho do Estudo (PICOS) direcionou a pesquisa. Resultados: Mulheres grávidas com HIV apresentaram alta prevalência de IST, incluindo Chlamydia trachomatis, Neisseria gonorrhoeae, Trichomonas vaginalis e vaginose bacteriana. A infecção por HIV parece influenciar o risco e a gravidade das infecções urogenitais. A gravidez aumentou o risco de IST, independentemente do status de HIV. Os parceiros masculinos também podem influenciar a presença de IST em mulheres grávidas, especialmente aquelas com HIV. Conclusão: A associação entre o status de HIV e as infecções urogenitais em mulheres grávidas indica a necessidade de rastreamento e cuidado adequado. A prevenção e o tratamento de IST em gestantes são essenciais para a saúde materno-infantil, independentemente do status de HIV

Fatores associados à exposição ao HIV em usuários de álcool / Factors associated with HIV exposure in alcohol users

Introdução: A prevalência de HIV é maior em Porto Alegre comparada ao restante do país. O abuso de álcool afeta o juízo crítico, sendo associado a comportamentos de risco que podem levar à contaminação pelo HIV. Objetiva-se analisar fatores associados à exposição ao HIV em alcoolistas com práticas sexuais de risco comparando com aqueles que se previnem.Métodos: Estudo transversal com 126 homens alcoolistas (HIV negativo), divididos em 2 grupos: Exposto Sexual (n = 42) e Pouco Exposto Sexual (n = 84), considerando uso de preservativo e número de parceiros sexuais.Resultados: A maioria dos sujeitos do grupo Exposto Sexual era solteiro, sem ocupação laboral, com histórico de moradia de rua e de relação com profissional do sexo, apresentavam maior preocupação com infecção por HIV (p < 0,05).Conclusão: Sugere-se o desenvolvimento e monitoramento de intervenções preventivas específicas, considerando as características do abuso de álcool e seu papel na transmissão do HIV.

Introduction: The HIV prevalence is higher in Porto Alegre compared to other capitals in Brazil. Alcohol abuse affects critical judgment, being associated with risky behaviors that can lead to HIV infection. Aim:To analyze factors associated with exposure to HIV in alcohol users, comparing those with risky sexual practices and those who use prevention methods.Methods: Cross-sectional study with 126 male alcohol users (HIV negative), divided into 2 groups: Sexually Exposed (n = 42) and Less Sexually Exposed (n = 84), considering condom use and number of sexual partners.Results: Most subjects in the Sexually Exposed group were single, without a job, with a history of homelessness and a relationship with a sexual worker, and were more concerned about HIV infection (p < 0.05).Conclusion: The development and monitoring of specific preventive interventions is suggested, considering the characteristics of alcohol abuse and its role in HIV transmission.

Sexually transmitted infections （STIs） and related factors among pregnant women with non-local household registration in Xuhui District， Shanghai / 上海预防医学

ObjectiveTo study the sexually transmitted infections （STIs） in pregnant women with non-local household registration in Xuhui District and analyze the risk factors for STIs. MethodsFrom April 2020 to March 2022， pregnant women with non-local household registration who received their first prenatal examination in a general hospital in Shanghai Xuhui District were selected to conduct a status survey of STIs. Logistic regression model was used to analyze the influencing factors of bacterial vaginitis and ureaplasma uaplasma infection. ResultsThe top three infection rates in the pregnant women were Ureaplasma urealyticum （13.2%）， bacterial vaginosis （9.8%） and mycotic vaginitis （4.7%）. Age between 25 and 35 years （aOR=0.53，95%CI： 0.28‒0.98） and monthly income ≥8 000 yuan （aOR=0.30，95%CI： 0.11‒0.82） were significantly correlated with ureaplasma uaplasma infection. Pregnancy number of 2 （aOR=4.95， 95%CI： 1.59‒15.46）， first sexual relationship occurred before marriage （aOR=2.83， 95%CI： 1.04‒7.74）， husband's alcoholism （aOR=3.83， 95%CI： 1.08‒13.56）， high school education （aOR=0.27， 95%CI： 0.08‒0.93）， and husband's travel history （aOR=0.30， 95%CI： 0.12‒0.79） were significantly correlated with bacterial vaginitis. ConclusionPregnant women with more gestation times， first sexual intercourse before marriage and husband with heavy drinking are more likely to be infected with bacterial vaginosis. Pregnant women with younger age and lower income are more likely to be infected with Ureaplasma urealyticum. Follow-up and monitoring should be strengthened in these groups.

Seropositivity of Chlamydia trachomatis & Toxoplasma gondii among male partners of infertile couples in Odisha, India: A facility based exploratory study

Chlamydia trachomatis (CT) is the most common curable sexually transmitted non-viral infection, which can cause urethritis, prostatitis, orchitis and epididymitis among males. Toxoplasma gondii, a protozoan causes toxoplasmosis among humans. These infections may lead to decreased fertility. Hence, this study was aimed to estimate the seropositivity of CT and T. gondii infection among the male partners of infertile couples in Odisha, India. In this facility-based cross-sectional study, first void urine, seminal fluid and blood sample were collected from 153 males attending infertility clinics including 74 healthy controls. Urine and seminal fluid were tested through polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay was used to detect immunoglobulin (Ig) G, IgA and IgM anti-Chlamydia and IgM anti-Toxoplasma antibodies through serum. The prevalence of CT was 1.26 per cent (95% CI: 0.03-6.85) as estimated from semen samples using PCR. We detected anti-Chlamydia antibodies IgM-four per cent [95% confidence interval (CI): 0.83-11.24]; IgA-28.16 per cent (95% CI: 18.13-40.09) and IgG-12.5 per cent (95% CI: 5.87-22.4) among participants. Anti-Toxoplasma antibodies IgM were observed in 27.63 per cent (95% CI: 17.98-39.08) of participants. None of the control samples were found positive. Overall seropositivity of CT and Toxoplasma infections is comparable, which suggests that greater attention is required for screening these infections at clinics, especially among infertile couples.

Linfogranuloma venéreo genital en un hombre que tiene sexo con hombre: reporte de caso no importado en Chile / Lymphogranuloma venerous genital in men who have sex with men: a non-imported case report in Chile

Resumen El linfogranuloma venéreo (LGV) es una infección de transmisión sexual (ITS) causada por Chlamydia trachomatis. En los últimos años, ha emergido principalmente en hombres que tienen sexo con hombres (HSH). Es frecuente su asociación con otras ITS como el virus de inmunodeficiencia humana (VIH) y la sífilis. Si bien el compromiso genital es la forma de presentación clásica, el síndrome anorrectal constituye el principal cuadro clínico en la actualidad. Presentamos el caso de un HSH con infección por VIH en terapia anti-retroviral, herpes genital recurrente y sífilis latente tratada, sin viajes recientes al extranjero, con adenopatías inguinales fistulizadas a piel, asociada a una úlcera genital dolorosa, sin síntomas anorrectales ni sistémicos, refractario a múltiples tratamientos antibacterianos y antivirales. El estudio con RPC de secreción de la fístula fue positiva a C. trachomatis. Se trató con doxiciclina por seis semanas, con buena respuesta clínica, sin complicaciones.

Abstract Lymphogranuloma venereum (LGV) is a sexually transmitted infection caused by Chlamydia trachomatis bacteria. In the past years, it has emerged as a relevant infectious agent, mainly affecting men who have sex with men (MSM), frequently associated with other sexually transmitted infections as human immunodeficiency virus (HIV) and syphilis. Even though genital lesions correspond to the classical presentation of LGV, nowadays anorectal syndrome is more frequently reported. We present a MSM patient, HIV infected, being treated with antiretroviral drugs, with a history of recurrent genital herpes, also recently treated for a syphilis in a latent stage. He had no recent trips. He referred inguinal fistulized enlarged lymph nodes, associated with a painful genital ulcer, he denied anorectal or systemic symptoms. He had been treated with multiple antibiotic and antiviral drugs, with no clinical response. Molecular studies from the content of the fistulized lymph node identified C. trachomatis. The patient received doxycycline for 6 weeks, showing good clinical response.

Aislamiento de Neisseria meningitidis en muestra anorectal en hombre que tiene sexo con hombre con uretritis y sifilis primaria / Isolation of Neisseria meningitidis in anorectal specimen in a men who have sex with men with uretritis and primary syphilis

Resumen Neisseria meningitidis es una bacteria gramnegativa asociada frecuentemente a enfermedades invasoras de elevada mortalidad. Si bien su reservorio natural es la nasofaringe humana, en los últimos años han aumentado los aislamientos de este agente en la mucosa anorectal, principalmente en hombres que tienen sexo con hombres (HSH). Presentamos el caso de un HSH con infección por VIH, que consultó por un cuadro de uretritis y sifilis primaria, en el cual se aisló N. meningitidis en una muestra anorectal. Fue tratado en forma empírica con ceftriaxona y azitromicina, realizándose un cultivo de control post-tratamiento que fue negativo. A pesar del aumento de las infecciones y colonizaciones anogenitales por N. meningitidis, se desconoce su rol como patógeno genital, en la transmisión de otras infecciones y la necesidad de esquemas terapéuticos específicos.

Abstract Neisseria meningitidis is a Gram-negative bacterium frequently associated with invasive diseases with high mortality. Although its natural reservoir is the human nasopharynx, in recent years there have been increasing reports of isolation of this agent in the anorectal mucosa, mainly in men who have sex with men (MSM). We present the case of an HIV-positive MSM who consulted for urethritis and primary syphilis, in which N. meningitidis was isolated in an anorectal specimen. He was treated empirically with ceftriaxone and azithromycin, and a post-treatment control culture was negative. Despite the increase in anogenital infections and colonization by N. meningitidis, its role is unknown as a genital pathogen and in the transmission of other infections and the need for specific therapeutic regimens.

Infecções sexualmente transmissíveis na população residente no projeto de assentamento rural Nova Amazônia, Brasil / Sexually transmitted infections in the population living in the rural settlement project Nova Amazonia, Brazil

O adoecimento pode revelar impactos e consequências das desigualdades sociais, econômicas e culturais. Avaliar a soroprevalência da infecção pelo HIV, Hepatites B e C e Sífilis e o comportamento sexual dos moradores do Projeto de Assentamento Nova Amazônia (PANA). Trata-se de estudo observacional, transversal, descritivo, quantitativo e qualitativo, envolvendo 246 moradores do PANA, realizado entre novembro de 2019 e setembro de 2020. Foi aplicado um questionário e realizados testes rápidos para HIV 1 e 2, Sífilis e Hepatites B e C. Foi possível observar a prevalência de 4,1% para infecções sexualmente transmissíveis, estando associada estatisticamente com ser do gênero masculino (p = 0,04), ter mais de 40 anos (p = 0,003) e ter se relacionado com mais de 10 parceiros sexuais ao longo da vida (p = 0,03). Devido à identificação de condutas sexuais de risco, como baixíssima adesão ao uso do preservativo, faz-se necessária a realização de diagnóstico precoce e monitoramento de novos casos.

The illness can reveals impacts and consequences of social, economic and cultural inequalities. To evaluate the seroprevalence of HIV infection, Hepatitis B and C and Syphilis and sexual behavior of residents of the Nova Amazônia Settlement Project (PANA). Observational, cross-sectional, descriptive, quantitative and qualitative study, involving 246 residents of PANA, carried out between November 2019 and September 2020. A questionnaire was applied and rapid tests were performed for HIV 1 and 2, Syphilis and Hepatitis B and C. A prevalence of 4.1% for sexually transmitted diseases was observed, statistically associated with being male (p = 0.04), being over 40 years old (p = 0.003) and having been related with more than 10 sexual partners throughout life (p = 0.03). Due to the identification of risky sexual behaviors, such as very low adherence to condom use, it is necessary to perform an early diagnosis and monitor new cases.

Forma necrótica de úlcera de Lipschütz / Necrotic type of Lipschütz ulcer

Resumen INTRODUCCIÓN: Las úlceras de Lipschütz son una causa infrecuente de úlcera genital, de alivio espontáneo y casi siempre benignas, aunque en algunas pacientes pueden dejar cicatrices genitales. Lo común es que aparezcan en mujeres jóvenes, antes del inicio de la vida sexual activa, en coincidencia con un cuadro catarral o pseudogripal. CASO CLÍNICO: Paciente de 18 años con una úlcera vulvar dolorosa, coincidente con un episodio de amigdalitis aguda y fiebre de 38 ºC. La paciente negó haber tenido relaciones sexuales. La úlcera alcanzó 3 a 4 cm, profunda, purulenta, con apertura del labio menor derecho y con importante componente necrótico. Para el control del dolor se le indicaron: corticoides, doxiciclina oral, crema con lidocaína y antiinflamatorios. Los análisis de laboratorio descartaron que se tratara de infección de trasmisión sexual. Al término del esquema terapéutico prescrito la evolución fue favorable, con desaparición de los síntomas, pero con una secuela: apertura del labio mayor derecho. CONCLUSIONES: La úlcera de Lipschütz es una causa infrecuente de úlcera vulvar. Su tratamiento consiste en el control de los síntomas y casi siempre se cura en el transcurso de 4 a 6 semanas, sin dejar lesiones. El diagnóstico solo puede establecerse luego de excluir otras causas más frecuentes de úlcera vulvar.

Abstract INTRODUCTION: Lipschütz ulcers are an infrequent cause of genital ulcer, of spontaneous relief and almost always benign, although in some patients they may leave genital scars. They usually appear in young women, before the onset of active sexual life, coinciding with a catarrhal or flu-like condition. CLINICAL CASE: 18-year-old female patient with a painful vulvar ulcer, coinciding with an episode of acute tonsillitis and fever of 38 ºC. The patient denied having had sexual intercourse. The ulcer was 3 to 4 cm, deep, purulent, with opening of the right labium minora and with a significant necrotic component. For pain control she was prescribed corticosteroids, oral doxycycline, lidocaine cream and anti-inflammatory drugs. Laboratory tests ruled out sexually transmitted infection. At the end of the prescribed therapeutic scheme the evolution was favorable, with disappearance of symptoms, but with a sequel: opening of the right labium majus. CONCLUSIONS: Lipschütz ulcer is a rare cause of vulvar ulcer. Its treatment consists of symptom control and it almost always heals within 4 to 6 weeks, leaving no lesions. The diagnosis can only be established after other more frequent causes of vulvar ulceration have been excluded.

Chlamydia trachomatis, virus herpes simple-2 y Neisseria gonohrroeae: prevalência y factores de riesgo en estudiantes / Chlamydia trachomatis, herpes simplex virus-2 and Neisseria gonorrhoeae: Prevalence and risk factors in students

RESUMEN Objetivo Determinar la prevalencia de Chlamydia trachomatis (CT), virus herpes simple-2 (VHS-2) y N. gonorrhoeae (NG) y los factores de riesgo relacionados con la infección, en un grupo de estudiantes universitarios de la ciudad de Medellín. Metodología Se realizó un estudio descriptivo en un grupo de 323 estudiantes universitarios. A través de un formulario en línea, se realizó el reporte de los factores de riesgo y síntomas relacionados con infecciones de transmisión sexual (ITS) y luego se determinó la presencia de anticuerpos IgG y IgM para CT y VHS-2 y se realizó una prueba de PCR-RT para detectar NG y CT. Resultados La frecuencia de IgG para CT fue del 13% y la positividad para IgM fue del 11,9%. La frecuencia de IgG para VHS-2 fue del 11,8% y la frecuencia de CT y NG por la prueba PCR-RT fue del 1,5% y del 0%, respectivamente. Los factores de riesgo más frecuentes fueron: vida sexual activa en el 96,9%, uso algunas veces o nunca del condón en un 75,2%. Reportaron que tenían secreción genital el 13,6% de los estudiantes; úlceras, el 2,8%; verrugas, el 5,3%; ardor al orinar, el 15,5%; ampollas, el 4,6%, y diagnóstico previo de una ITS, el 18,9% de los estudiantes. Conclusión Se encontró CT y VHS-2 entre los jóvenes estudiados y además una alta frecuencia de factores de riesgo para la adquisición de ITS. Se recomienda mejorar las campañas de prevención y diagnóstico de las ITS en los jóvenes universitarios.

ABSTRACT Objective The aim of this study was to determine the prevalence of Chlamydia trachomatis (CT), Herpes Simple-2 virus (HSV-2) and N. gonorrhoeae (NG), and the risk factors related to the infection in a group of college students in the city of Medellín, Colombia. Methods A descriptive study was carried out in a group of 323 university students. Through an online form, the risk factors and symptoms related to sexually transmitted infection (STI) were reported, and then the presence of IgG and IgM antibodies for CT and HSV-2 was determined, and RT-PCR testing was performed to detect NG and CT. Results 13% was the IgG frequency for CT while 11.9% was for IgM. The frequency for IgG for VHS-2 was 11.8 while the frequencies determined by RT-PCR for CT and NG were 1.5% and 0.0% respectively. The risk factors most commonly associated were active sex life, 96.9%, and random use of condom, 75.2%. It was also found that 13.6% of the students had genital secretions, 2.8% had ulcers, 5.3% had genital warts, 4.6% had blisters, 15.5% had dysuria, and 18.9% had a previous STI diagnosis. Conclusions CT and HVS-2 were found among college students, besides a series of risk factors associated with STI. It is recomended to improve prevention and diagnosis campaigns among young college students.

Actualización de la infección por Chlamydia trachomatis en mujeres / Update of Chlamydia trachomatis infection in women

La infección por Chlamydia trachomatis es la infección de transmisión sexual bacteriana más frecuente en el mundo. En este artículo se revisa la prevalencia reportada en Chile, cuadro clínico, diagnóstico, tratamiento, prevención y complicaciones a largo plazo en mujeres.

Chlamydia trachomatis infection is known to be the most common sexually transmitted bacterial infection world-wide. This article reviews the prevalence reported in the Chilean population, as well as the clinical manifestations, diagnosis, treatment, prevention and complications in women

mHealth na prevenção das Infecções Sexualmente Transmissíveis (IST) / mHealth in the prevention of sexually transmitted infections STIs

Resumo O objetivo deste estudo foi analisar na literatura como os aplicativos foram elaborados e como influenciam na prevenção das Infecções Sexualmente Transmissíveis (IST). Levantou-se nas bases de dados PubMed, Periódicos da CAPES e Bireme utilizando os descritores "sexually transmitted diseases" e "mHealth" combinados pelo conector booleano AND. Considerou-se o período entre 2013 e 2017, artigos disponibilizados "on-line" em português e inglês. Os resultados dos estudos mostraram que para a elaboração do aplicativo a pesquisa formativa prevaleceu como método e o grupo focal como a técnica para a coleta de dados. Essa técnica objetiva a troca de experiencia e oportuniza a discussão de assuntos inerentes às infecções. Considera-se que os aplicativos foram construídos com rigor metodológico com a participação de usuários e com recursos instrucionais que influenciam o manejo da prevenção e o controle das infecções.

Abstract The objective of this study was to analyze in the literature how the applications were developed and how they influence the prevention of Sexually Transmitted Infections (STIs). PubMed, CAPES Periodicals and Bireme databases were searched using the descriptors sexually transmitted diseases and mHealth combined by the Boolean connector AND. The search considered studies published between 2013 to 2017 available online in Portuguese and English. The results of the studies showed formative research was the most used method and focus group was the most used technique for data collection in the development of an application. This technique aims at the exchange of experience and allows discussion about issues inherent to infections. The applications were built with methodological rigor with the participation of users and with instructional resources that influence the management of the prevention and control of infections.

Incidência de Chlamydia trachomatis em gestantes diagnosticadas com sífilis / Chlamydia trachomatis incidence on syphilis diagnosed pregnant women

De acordo com a Organização Mundial da Saúde, a cada ano, mais de 376 milhões de pessoas são infectadas pelas doenças sexualmente transmissíveis, das quais 127,2 milhões são por Chlamydia trachomatis. As infecções genitais não tratadas na gestação, principalmente as assintomáticas, podem acarretar complicações, tais como: endometrite puerperal e síndrome de Fitz-Hugh-Curtis. A pesquisa científica foi realizada com o objetivo de identificar a presença de Chlamydia trachomatis na secreção endocervical de gestantes sifilíticas, estabelecendo o perfil das gestantes a partir de informações dos possíveis sinais e sintomas durante a gravidez e hábitos gestacionais. Adotou-se como caminho metodológico a pesquisa quantitativa, descritiva, bibliográfica e laboratorial. Como resultados finais, 54% das gestantes foram diagnosticadas com Chlamydia trachomatis e 67% eram assintomáticas. Correlacionamos também que as altas taxas de infecção estão associadas com a falta de conhecimento sobre infecções sexualmente transmissíveis e suas formas de prevenção.(AU)

According to the World Health Organization, each year, more than 376 million people are infected with sexually transmitted diseases, of which 127.2 million are Chlamydia trachomatis, it is a asymptomatic disease, meaning there are no symptoms. Genital infections can lead to complications such as: puerperal endometritis and Fitz-Hugh- -Curtis syndrome. The scientific research was carried out to identify the presence of Chlamydia trachomatis in the endocervical secretion of syphilitic pregnant women. This research established a profile of pregnant women infected with Chlamydia trachomatis using information about possible signs and symptoms during pregnancy and gestational habits. It provides a methodological way to quantify and describe the laboratory research. The results showed, 54% of the pregnant women were diagnosed with Chlamydia trachomatis of which 67% were asymptomatic. We also correlate that high rates of Chlamydia trachomatis infection are associated with lack of knowledge about sexually transmitted infections and their forms of prevention.(AU)

A study of health seeking behavior about sexually transmitted infection in women with high risk behavior in an urban slum

Background: Health care seeking for sexually transmitted infection is frequently inadequate, particularly among female sex workers because of the low levels of awareness regarding sexual health. Correct diagnosis and treatment with health information are essential components of sexually transmitted infection (STI) services. Objective was to study demographic profile and to assess their health seeking behavior for STI and prevention services.Methods: Study was done in urban slum in Mumbai. It was cross sectional study conducted from May 2018 to December 2018.Results: The median age of women was 26 years. Majority belong to lower socio economic class, 39.4% women admitted that they took alcohol on occasions while 22% had sex with alcohol intake, 41% women were using condom during sexual activity. Only 14.5% women completed treatment who had past history of STI.Conclusions: Ensuring timely complete treatment of STI/RTI among women with high risk behavior is priority to prevent spread among general population.

Concepciones sobre infecciones de transmisión sexual en estudiantes universitarios de pregrado / Conceptions on sexual transmission infections in undergraduate university students

Introducción: Actualmente se ha incrementado el número de personas que contraen una Infección de Trasmisión Sexual (ITS) por el aumento en el número de parejas sexuales a lo largo de la vida y por la carencia de formación adecuada. Dado que, los principales afectados por las ITS son los jóvenes, el objetivo: de esta investigación consistió en identificar las concepciones sobre ITS en estudiantes universitarios de pregrado. Materiales y métodos: En este estudio cualitativo con enfoque exploratorio descriptivo se empleó una encuesta estructurada y auto-administrada aplicada a 74 estudiantes. Resultados: Mediante estadística descriptiva e inferencial se evidenció que hay confusiones respecto a las vías de contagio, formas de protección, claridad de términos, probabilidad de contraer una infección según el género y desarrollo de las ITS y, básicamente no existen diferencias estadísticamente significativas entre las concepciones de los estudiantes del primer y último semestre (P>0.05). Se encontró también, que los padres (47%) y profesores (41%) se constituyen como las principales fuentes de información sobre ITS a través de las cuales los estudiantes tienen su primera orientación educativa respecto al tema y, a pesar del paso por la academia, pareciera que esta no transforma en buena medida aquellos conocimientos errados de sexualidad que muchas veces se traen de casa o de la escuela. Conclusión: Se sugieren pautas y estrategias desde la Universidad que permitirán esclarecer y fortalecer las concepciones sobre las ITS en los docentes en formación en particular y, a la población universitaria en general.

Introduction: At present, the number of people getting a Sexually Transmitted Infection (STIs) has increased due to the growing number of sexual couple along life and because of the lack of the right education. Given that, the main affected by STIs are young people, the objective: of this research was to identify the conceptions of STIs in undergraduate university students. Material and Methods: In this qualitative study with a descriptive exploratory approach, a structured and self-administered survey applied to 74 students was used. Results: By means of descriptive and inferential statistics it was shown that there are confusions regarding the routes of contagion, forms of protection, clarity of terms, probability of contracting an infection according to gender and development of STIs, and basically there are no statistically significant differences between conceptions of the students of the first and last semester (P> 0.05). It was found too that the parents (47%) and teachers (41%) were constituted as the principal sources of information about STIs through the students have their first sexual orientation about this topic and, in spite their time in academy looks like it does not change in a good way those wrong knowledge of sexuality that usually are taken from home o school. Conclusion: guidelines and strategies are suggested from the University that will clarify and strengthen the conceptions of STIs in teachers in training in particular and the university population in general.

Website sobre sexualidade e prevenção de infecções sexualmente transmissíveis para adolescentes católicos / Sitio web sobre sexualidad y prevención de infecciones de transmisión sexual para adolescentes católicos / Website on sexuality and prevention of Sexually Transmitted Infections for Catholic teenagers

Resumo Objetivo Desenvolver e avaliar conteúdo e interface de website sobre sexualidade e prevenção de infecção sexualmente transmissível para adolescentes católicos. Métodos Pesquisa de desenvolvimento metodológico e tecnológico, cujas etapas de avaliação do website foram: desenvolvimento, avaliação e distribuição. O desenvolvimento contemplou as fases de análise e planejamento; modelagem; implementação e avaliação do website. A avaliação consistiu no julgamento de 19 participantes, sendo dez juízes em conteúdo, quatro da área de computação e design, e cinco adolescentes. Por fim, houve a distribuição para acesso on-line gratuito. Resultados Foi desenvolvido o website denominado Papo de Adolescente, que abordou os conteúdos religião, adolescência, sexualidade, Infecções Sexualmente Transmissíveis/HIV/AIDS, prevenção e principais dúvidas. O índice geral de avaliação do conteúdo atribuído pelos juízes conhecedores do tema foi 0,88 e, pelos juízes da área de computação e desing, foi 0,70. Após a análise, sugestões e readequações foram incorporadas ao website, como a reorganização do design e dos conteúdos sobre adolescência, sexualidade e Infecções Sexualmente Transmissíveis/HIV/AIDS, além da reescrita do texto sobre Infecções Sexualmente Transmissíveis/HIV/AIDS, referente aos critérios autoria, primeira impressão, facilidade de navegação e uso dos gráficos. O website foi avaliado pelos adolescentes, que atribuíram avaliação geral de 0,94. Conclusão O website apresentou avaliação de seu conteúdo e interface adequados, para abordar sexualidade e prevenção das Infecções Sexualmente Transmissíveis com adolescentes católicos.

Resumen Objetivo Desarrollar y evaluar el contenido e interfaz de un sitio web sobre sexualidad y prevención de infecciones de transmisión sexual para adolescentes católicos. Métodos Investigación de desarrollo metodológico y tecnológico, cuyas etapas de evaluación del sitio web fueron: desarrollo, evaluación y distribución. El desarrollo contempló la fase de análisis y planificación, modelado, implementación y evaluación del sitio web. La evaluación consistió en la apreciación de 19 participantes, de los cuales 10 eran jueces de contenido, 4 del área de computación y diseño y 5 adolescentes. Por último, se realizó la distribución para el acceso en línea gratuito. Resultados Se desarrolló el sitio web denominado Papo de Adolescente (Charla de Adolescentes), que abordó contenidos sobre religión, adolescencia, sexualidad, infecciones de transmisión sexual/VIH/SIDA, prevención y las principales dudas. El índice general de evaluación del contenido adjudicado por los jueces conocedores del tema fue de 0,88 y por los jueces del área de computación y diseño, 0,70. Luego del análisis, se incorporaron sugerencias y se realizaron adaptaciones en el sitio web, como la reorganización del diseño y de los contenidos sobre adolescencia, sexualidad e infecciones de transmisión sexual/VIH/SIDA, además de reescribir el texto sobre infecciones de transmisión sexual/VIH/SIDA, con relación a los criterios autoría, primera impresión, facilidad de navegación y uso de los gráficos. El sitio web fue evaluado por los adolescentes, que le adjudicaron una evaluación general de 0,94. Conclusión El sitio web presentó una evaluación de contenido e interfaz adecuada para abordar la sexualidad y la prevención de las infecciones de transmisión sexual con adolescentes católicos.

Abstract Objective To develop and assess content and interface of website on sexuality and sexually transmitted infection prevention for Catholic teenagers. Methods A research on methodological and technological development, whose stages of assessment on the website were: development, assessment and distribution. The development included the analysis and planning phases; modeling; establishment and assessment of the website. The assessment consisted of the judgment of 19 participants, with ten judges in content, four in the area of computing and design, and five teenagers. Finally, there was distribution for online free access. Results The website Papo de Adolescente (Teen Chat) was developed, which addressed the contents of religion, adolescence, sexuality, Sexually Transmitted Infections/HIV/AIDS, prevention and main doubts. The overall content rating index attributed by judges who knew the topic was 0.88 and, by the judges in the area of computing and design, 0.70. After the analysis, suggestions and readjustments were incorporated into the website, such as the reorganization of the design and the contents on adolescence, sexuality and Sexually Transmitted Infections/HIV/AIDS, in addition to the rewriting of the text on Sexually Transmitted Infections/HIV/AIDS regarding the criteria of authorship, first impression, ease of navigation and use of graphics. The website was rated by teenagers, who rated it as 0.94 overall. Conclusion The website presented an assessment of its content and adequate interface, to address sexuality and prevention of Sexually Transmitted Infections with Catholic teenagers.

Association between asymptomatic sexually transmitted infections and high-risk human papillomavirus in cervical lesions / 西安交通大学学报(医学版)

Objective To determine the association of asymptomatic sexually transmitted infections (STIs) with high-risk human papillomavirus (hrHPV) in cervical squamous intraepithelial lesions and neoplasms. Methods From July 2017 to July 2018, 320 hrHPV-positive and 160 hrHPV-negative women in The First Affiliated Hospital of Xi'an Jiaotong University were divided into normal+low-grade squamous intraepithelial lesion (LSIL) and high-grade squamous intraepithelial lesion (HSIL)+ invasive cervical cancer (ICC) subgroups, respectively, based on their pathological cervical lesion grades. Cervical brush specimens including Ureaplasma urealyticum (UU) and its serotypes, Mycoplasma hominis (MH), Mycoplasma genitalium (MG), Chlamydia trachomatis (CT), and hrHPV were amplified and hybridized using PCR kits (Hybribio Biochemistry Co., Ltd.). The differences between groups were examined by the chi-squared test, continuity correction and Logistic regression. Results The overall infection (at least one of the non-hrHPV pathogens was positive) rates of the two groups were 62.5% and 59.4%, which were not significantly different. The prevalence of UU was the highest. The prevalence of UU serotype 14 (Uup14) and MH was associated with hrHPV (P=0.003, P=0.005, respectively), but not with certain hrHPV genotypes or with single or multiple genotypes. Compared with cases of normal+LSIL, cases of HSIL+ICC had significantly different Uup14 infection rates (OR: 12.579, 95% CI: 3.638-43.497, P<0.001) in the positive group. In hrHPV-negative cases, there were obvious differences in Uuu and Uup1 infections based on different cervical lesion types (OR: 11.646, 95% CI: 1.493-90.850, P=0.019; OR: 7.474, 95% CI: 1.140-49.015, P=0.036). Conclusion Asymptomatic STIs in female lower reproductive tract are widespread. Uup14 may increase the risk of HSIL+ICC when hrHPV is positive. Uuu and Uup1 cause an increased risk of HSIL+ICC without hrHPV.

Evaluation of Multiplex Polymerase Chain Reaction Assay for the Simultaneous Detection of Sexually Transmitted Infections Using Swab Specimen

Sexually transmitted infections (STIs) are caused by the spread of pathogens via sexual activity and can cause serious complications if left untreated, regardless of their symptoms. Therefore, early diagnosis of STI is important, and molecular diagnostic methods for rapid detection and monitoring are needed. In this study, we evaluated a multiplex polymerase chain reaction (PCR) kit for simultaneously detecting 13 different bacterial, fungal, and viral microorganisms that cause STIs. The kit performance was evaluated for its sensitivity, lot-to-lot variation, and interference in detecting different pathogens. Additionally, its clinical usefulness was evaluated by estimating its sensitivity and specificity for clinical samples. The limit of detection (LOD) was 0.021–50.104 copies for each pathogen. In the tests of lot-to-lot, 100% of positive samples were detected at low concentrations and negative samples all showed negative results. This result confirms that there is no the variation of lot-to-lot. In the test for interference between pathogens, the efficiency of amplification for each pathogen was not significantly reduced and no nonspecific amplification product was formed. We tested 322 vaginal swab samples using the multiplex PCR kit and confirmed that its clinical sensitivity and specificity were 100% for all pathogens. This multiplex PCR kit can be used widely for rapid diagnosis and monitoring of STIs.